Abstract
Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit angiogenesis. We showed that treatment with 15d-PGJ(2), a PPARgamma ligand, downregulate the expressions of angiopoietin-1 (Ang-1) in gastric cancer cells MKN45. The medium of MKN45 cells treated with 15d-PGJ(2) significantly inhibited the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Moreover, Matrigel plug assay revealed that 15d-PGJ(2) reduced in vivo angiogenesis induced by MKN45 cells. These modulations were restored by the addition of recombinant Ang-1. Our findings supported that 15d-PGJ(2) suppressed angiogenesis of gastric cancer cells by downregulation of Ang-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiogenesis Inhibitors / pharmacology
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Angiopoietin-1 / genetics*
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Animals
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Cell Line
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Down-Regulation / drug effects
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Down-Regulation / genetics
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Endothelial Cells / cytology
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Endothelial Cells / drug effects
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Endothelial Cells / physiology
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Female
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Gene Expression Profiling
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neovascularization, Pathologic / genetics
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Neovascularization, Pathologic / metabolism
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Neovascularization, Pathologic / prevention & control*
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Neovascularization, Physiologic / drug effects
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Oligonucleotide Array Sequence Analysis
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Prostaglandin D2 / analogs & derivatives*
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Prostaglandin D2 / pharmacology
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Stomach Neoplasms / blood supply*
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Stomach Neoplasms / pathology
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Vascular Endothelial Growth Factor A / metabolism
Substances
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15-deoxyprostaglandin J2
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Angiogenesis Inhibitors
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Angiopoietin-1
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Vascular Endothelial Growth Factor A
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Prostaglandin D2