Abstract
The VraS/VraR two-component system (VraSR) regulates transcriptional induction of penicillin-binding protein 2 (encoded by pbp2) by vancomycin in Staphylococcus aureus. We have now defined the vraSR operon and determined that its induction by beta-lactams as well as by vancomycin is autoregulated. Induction of the pbp2 and vraSR operons by beta-lactams and related compounds within 1 hour after exposure to the antimicrobials was dependent on vraS. However, when a disk diffusion assay that can detect induction of genes over an extended time period was used, induction of the pbp2 operon was mediated by some beta-lactams, including oxacillin; this induction was independent of vraS.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / metabolism*
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Cell Wall / chemistry*
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DNA-Binding Proteins
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Drug Resistance, Multiple, Bacterial / genetics
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Gene Expression Regulation, Bacterial / drug effects
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Gene Expression Regulation, Bacterial / physiology*
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Microbial Sensitivity Tests
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Operon
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Penicillin-Binding Proteins
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Signal Transduction
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Staphylococcus aureus / chemistry
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Staphylococcus aureus / metabolism*
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Staphylococcus aureus / physiology
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Vancomycin / pharmacology
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Penicillin-Binding Proteins
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VraR protein, Staphylococcus aureus
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VraS protein, Staphylococcus aureus
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Vancomycin