Effects of temperature, light density, nitrogen level and phosphorus level of medium to the content of two species Spirodela polyrrhiza and Lemna aequinoctialis were researched. The results showed that the content of nitrogen in two species kept constant when the concentration of nitrogen was higher than 3 mg x L(-1) and temperature higher than 25 degrees C. It decreased under lower concentrations of nitrogen and lower temperature. Photon flux density had no effect on it. But weak photon flux density and low temperature led to reduce the content of phosphorus of duckweed. The content of phosphorus in two duckweed species kept constant when photon flux density was higher than 8 000 1x and temperature higher than 25 degrees C. The relationship between the content of phosphorus and concentrations of phosphorus in medium could be described by Monod model. The saturated phosphorus content of Spirodela polyrrhiza was higher than Lemna aequinoctialis.