Intein-mediated, in vitro and in vivo protein modifications with small molecules

Protein Pept Lett. 2005 Nov;12(8):769-75. doi: 10.2174/0929866054864193.

Abstract

We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biotin / chemistry
  • Biotinylation / methods
  • Cell-Free System
  • Green Fluorescent Proteins / chemistry
  • Inteins / physiology*
  • Protein Array Analysis / methods
  • Protein Engineering / methods
  • Protein Modification, Translational / physiology*
  • Proteins / chemistry*
  • Proteomics / methods

Substances

  • Proteins
  • Green Fluorescent Proteins
  • Biotin