Abstract
GB virus C (GBV-C) is a common, apparently nonpathogenic human flavivirus that has been associated in some studies with prolonged survival in human immunodeficiency virus (HIV)-positive persons. There are 5 distinct GBV-C genotypes localized to specific geographic regions, and genotype 2 has been previously shown to inhibit HIV replication in vitro in lymphocyte cultures. We identified GBV-C virus isolates representing genotypes 1, 2, and 5 in samples from South African blood donors. GBV-C genotype 1 and 5 isolates replicated in lymphocyte culture, inhibited X4 and R5 HIV-1 isolates, and induced RANTES and stromal-derived factor-1 chemokines in vitro. Thus, African GBV-C genotypes can inhibit HIV replication in vitro.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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5' Untranslated Regions / chemistry
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5' Untranslated Regions / genetics
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Blood Donors
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Cells, Cultured
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Chemokine CCL5 / analysis
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Chemokine CXCL12
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Chemokines, CXC / analysis
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Flaviviridae Infections / epidemiology*
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Flaviviridae Infections / virology
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GB virus C / classification*
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GB virus C / genetics
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GB virus C / isolation & purification
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GB virus C / physiology*
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HIV Core Protein p24 / analysis
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HIV-1 / physiology*
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Humans
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Leukocytes, Mononuclear / virology
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Male
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Phylogeny
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Sequence Analysis, DNA
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South Africa / epidemiology
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Viral Interference*
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Viremia / epidemiology
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Virus Replication
Substances
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5' Untranslated Regions
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CXCL12 protein, human
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Chemokine CCL5
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Chemokine CXCL12
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Chemokines, CXC
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HIV Core Protein p24