Single nucleotide polymorphism detection in promoter III of the acetyl-CoA carboxylase-alpha gene in sheep

J Anim Breed Genet. 2005 Dec;122(6):418-20. doi: 10.1111/j.1439-0388.2005.00550.x.

Abstract

Three novel SNPs were identified in the locus OAR292286, encoding the DNA sequence of promoter III of the ovine acetyl-CoA carboxylase-alpha gene, in Italian sheep of four breeds: Gentile di Puglia (25 individuals) and Sopravissana (31) which are triple-purpose local endangered breeds, Comisana (25) which is a local non-selected, non-endangered dairy breed and Sarda (15) which is a popular selected high yielding dairy breed. Variant alleles are: G/T at 1330 bp, C/G at 1338 bp and C/T at 1430 bp. Frequencies of the variant alleles were calculated and chi-squared analysis of the differences in allele frequency between breed pairs was performed. Allele frequencies of the Sarda breed differ significantly from the other considered breeds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / genetics*
  • Animals
  • Breeding / methods*
  • Chromatography, High Pressure Liquid
  • DNA Primers
  • Gene Frequency
  • Italy
  • Polymorphism, Single Nucleotide / genetics*
  • Promoter Regions, Genetic / genetics
  • Sheep / genetics*

Substances

  • DNA Primers
  • Acetyl-CoA Carboxylase