Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis

Appl Environ Microbiol. 2005 Nov;71(11):7607-9. doi: 10.1128/AEM.71.11.7607-7609.2005.

Abstract

Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.

Publication types

  • Evaluation Study

MeSH terms

  • Bacillus subtilis / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli Proteins
  • Genes, Bacterial*
  • Genetic Techniques
  • MutS DNA Mismatch-Binding Protein / genetics
  • MutS DNA Mismatch-Binding Protein / metabolism
  • Plasmids / genetics*
  • Recombination, Genetic*

Substances

  • Escherichia coli Proteins
  • MutS DNA Mismatch-Binding Protein
  • MutS protein, E coli