Aim: To construct adenovirus expressing secretory human CD40L-Ig (sCD40 ligand Ig fusion protein).
Methods: The genes encoding the extracellular domain of human CD40L and IgG Fc were amplified with PCR and inserted into shuttle vector pAdTrack-CMV which then was transformed into E. coli pAdEasy-1-BJ5183 to produce recombinant Ad plasmid. The recombinant plasmid was digested with Pac I and transfected into 293 cells to generate recombinant adenovirus. The recombinant adenovirus was then used in mixed lymphocyte reaction (MLR) to test its function.
Results: The recombinant adenovirus sCD40L-Ig was produced. It could inhibit the lymphocyte proliferation in MLR.
Conclusion: The sCD40L-Ig adenovirus is prepared successfully and its inhibition of MLR is confirmed.