Electrophoretic variants for seven isozyme systems - probably encoded by 18 structural gene loci - in diploid populations of Larrea divaricata and diploid and tetraploid populations of its North American vicariant derivative L. tridentata were assayed by polyacrilamide and starch gel electrophoresis. High molecular similarity of diploid and tetraploid cytotypes of L. tridentata supports the hypothesis of interracial autopolyploidy. The absence of fixed heterozygosity and additive profiles indicates a low level of divergence between the parental diploids and the tetraploids. The phenogram based on the I coefficient showed the similarities between the populations of diploid L. divaricata and also between the diploid populations of L. tridentata. Both groups of diploid populations were more distantly connected to tetraploid L. tridentata.