Genomic cloning and characterization of a novel lectin gene from Zantedeschia aethiopica

Biosci Rep. 2004 Jun;24(3):225-34. doi: 10.1007/s10540-005-2583-4.

Abstract

A new lectin gene was isolated by using genomic walker technology and revealed to encode a mannose-binding lectin. Analysis of a 2233 bp segment revealed a gene including a 1169 bp 5' flanking region, a 417 bp open reading frame (ORF) and a 649 bp 3' flanking region. There are two putative TATA boxes and eight possible CAAT boxes lie in the 5' flanking region. The ORF encodes a 15.1 kDa precursor, which contains a 24-amino acid signal peptide. One possible polyadenylation signal is found in the 3'-flanking region. No intron was detected within the region of genomic sequence corresponding to zaa (Zantedeschia aethiopica agglutinin) full-length cDNA, which is typical of other mannose-binding lectin gene that have been reported. The deduced amino acid sequence of the lectin gene coding region shares 49-54% homology with other known lectins. The cloning of this new lectin gene will allow us to further study its structure, expression and regulation mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Lectins / genetics*
  • Lectins / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Sequence Alignment
  • Zantedeschia / chemistry*
  • Zantedeschia / genetics*

Substances

  • Lectins
  • Plant Proteins