Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

Opt Lett. 2005 Sep 1;30(17):2266-8. doi: 10.1364/ol.30.002266.

Abstract

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complexbiomolecular dynamics on a single molecule level.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Feasibility Studies
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Image Interpretation, Computer-Assisted / methods
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Refractometry / instrumentation*
  • Refractometry / methods
  • Spectrometry, Fluorescence / instrumentation*
  • Spectrometry, Fluorescence / methods