Abstract
A gene coding for a hypothetical membrane protein from Bifidobacterium breve was expressed in Lactococcus lactis. Immunoblotting demonstrated that this protein is located in the membrane. Phenotypical changes in sensitivity towards 21 antibiotics were determined. The membrane protein-expressing cells showed higher levels of resistance to several macrolides.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Bifidobacterium / genetics
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Bifidobacterium / metabolism*
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Computational Biology
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Drug Resistance, Bacterial*
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Genes, Bacterial
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Genome, Bacterial
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Lactococcus lactis / genetics
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Macrolides / pharmacology*
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Membrane Proteins / metabolism*
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Microbial Sensitivity Tests
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Macrolides
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Membrane Proteins