Purpose: Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB).
Methods: Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers.
Results: The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity.
Conclusion: The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.