Trapping of wide range mass-to-charge ions and dependence on matrix amount in internal source MALDI-FTMS

J Am Soc Mass Spectrom. 2005 Nov;16(11):1772-80. doi: 10.1016/j.jasms.2005.06.014. Epub 2005 Sep 22.

Abstract

Internal ionization source MALDI-FTMS shows clear variation of number average molecular weight (M(n)) for an equimolar mixture of four PEG polymers (PEG 2000, PEG 4000, PEG 6000, PEG 8000) when the 2,5-dihydroxy benzoic acid (DHB) matrix to PEG ratio is varied or the laser power is changed. As the matrix to analyte ratio is increased, the analyte signal of higher molecular mass ions increases and the signal of lower mass ions decreases. Laser power dependence studies show a similar trend. Possible explanations for these observations are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms*
  • Benzoates / chemistry*
  • Ions
  • Molecular Weight
  • Polyethylene Glycols / analysis*
  • Polyethylene Glycols / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Static Electricity

Substances

  • Benzoates
  • Ions
  • Polyethylene Glycols