Pseudo-storage pool disease due to platelet degranulation in EDTA-collected peripheral blood: a rare artifact

Clin Lab Haematol. 2005 Oct;27(5):336-42. doi: 10.1111/j.1365-2257.2005.00719.x.

Abstract

We report a patient who was referred for acute myocardial infarction and presented a pseudo-storage pool disease. The platelets from the blood collected with ethylene diamine tetra-acetic acid (EDTA) were moderately underestimated by the automated analyser, stained poorly on the blood smear and appeared agranular under the microscope. This artifactual anomaly does not occur in samples anticoagulated with citrate, heparin or the mixture citrate theophyline adenine dipyridamole. Reports of EDTA-induced pseudo-storage pool disease are scarce, possibly because underestimation of this poorly explained and difficult to diagnose phenomenon.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Blood Platelets / pathology*
  • Blood Specimen Collection / methods
  • Blood Specimen Collection / standards*
  • Cell Degranulation / drug effects*
  • Diagnostic Errors*
  • Edetic Acid / pharmacology*
  • Female
  • Histocytochemistry / standards
  • Humans
  • Myocardial Infarction / blood
  • Platelet Count
  • Platelet Storage Pool Deficiency / diagnosis*

Substances

  • Edetic Acid