Differentiation of a passive vaccine and the humoral immune response toward infection: analysis of phage displayed peptides

Vaccine. 2006 Jan 30;24(5):607-12. doi: 10.1016/j.vaccine.2005.08.039. Epub 2005 Aug 24.

Abstract

Antibody-genes undergo molecular events that produce unique binding-sites that recognize specific epitopes, thus, leading to B-cell clonal variation. As a result, different binding-site structures (paratope internal images) are produced even when two distinct B-cells bind one and the same epitope. Paratope structural variation can be exploited to enable one to evaluate antibody-diversity in a single polyclonal serum sample. This is accomplished through the selection of antibody-specific peptides isolated from combinatorial phage displayed peptide libraries. As an example, we demonstrate the analysis of macaque sera containing passively administered antibodies, given as a therapeutic vaccine and antibodies actively produced by the virus-infected monkeys.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation / immunology*
  • Bacteriophages / immunology
  • Cross Reactions
  • Epitope Mapping
  • Immunization, Passive*
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / genetics
  • Macaca mulatta
  • Peptide Library*
  • Reverse Transcriptase Polymerase Chain Reaction
  • SAIDS Vaccines / immunology
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control

Substances

  • Immunoglobulin G
  • Peptide Library
  • SAIDS Vaccines