Enhancing immune responses against a plasmid DNA vaccine encoding a FMDV empty capsid from serotype O

Yanmin Li; Neeraj Aggarwal; Haru-H Takamatsu; Catrina M A Sterling; Charlotte Voyce; Paul V Barnett

doi:10.1016/j.vaccine.2005.08.032

Enhancing immune responses against a plasmid DNA vaccine encoding a FMDV empty capsid from serotype O

Vaccine. 2006 May 22;24(21):4602-6. doi: 10.1016/j.vaccine.2005.08.032. Epub 2005 Aug 24.

Authors

Yanmin Li¹, Neeraj Aggarwal, Haru-H Takamatsu, Catrina M A Sterling, Charlotte Voyce, Paul V Barnett

Affiliation

¹ FMD Vaccine Group, Pirbright Laboratory, Institute for Animal Health, Ash Road, Woking, Surrey GU24 0NF, UK.

PMID: 16154238
DOI: 10.1016/j.vaccine.2005.08.032

Abstract

Various parameters have been examined for the improvement of immune responses induced by immunisation with foot-and-mouth disease virus (FMDV) DNA vaccine (pcDNA 3.1/P1-2A3C3D, P1) in swine. Our findings show that increasing the amount of P1 DNA plasmids and pGMCSF adjuvant plasmids induces stronger FMDV specific and neutralising antibody responses, as well as promoting cytokines IL-8 and IFNgamma secretion, in immunised pigs via multiple inoculation sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / blood
Capsid*
Cytokines / biosynthesis
Foot-and-Mouth Disease Virus / immunology*
Neutralization Tests
Plasmids*
Vaccines, DNA / immunology*

Substances

Antibodies, Viral
Cytokines
Vaccines, DNA