Method for isolation of Bacteroides bacteriophage host strains suitable for tracking sources of fecal pollution in water

Appl Environ Microbiol. 2005 Sep;71(9):5659-62. doi: 10.1128/AEM.71.9.5659-5662.2005.

Abstract

Bacteriophages infecting Bacteroides are potentially a good tool for fecal source tracking, but different Bacteroides host strains are needed for different geographic areas. A feasible method for isolating Bacteroides host strains for phages present in human fecal material is described. Useful strains were identified for application in Spain and the United Kingdom. One strain, GA-17, identified as Bacteroides thetaiotaomicron, was tested in several locations in Europe with excellent performance in Southern Europe.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage Typing
  • Bacteriophages / isolation & purification*
  • Bacteriophages / physiology
  • Bacteroides / classification*
  • Bacteroides / isolation & purification
  • Bacteroides / virology
  • Environmental Monitoring / methods*
  • Europe
  • Feces / microbiology*
  • Feces / virology
  • Humans
  • Species Specificity
  • Water Pollution*