Disintegration of Staphylococcus epidermidis biofilms under glucose-limiting conditions depends on the activity of the alternative sigma factor sigmaB

Sebastian Jäger; Dietrich Mack; Holger Rohde; Matthias A Horstkotte; Johannes K-M Knobloch

doi:10.1128/AEM.71.9.5577-5581.2005

Disintegration of Staphylococcus epidermidis biofilms under glucose-limiting conditions depends on the activity of the alternative sigma factor sigmaB

Appl Environ Microbiol. 2005 Sep;71(9):5577-81. doi: 10.1128/AEM.71.9.5577-5581.2005.

Authors

Sebastian Jäger¹, Dietrich Mack, Holger Rohde, Matthias A Horstkotte, Johannes K-M Knobloch

Affiliation

¹ Universitätsklinikum Hamburg-Eppendorf, Zentrum für Klinisch-Theoretische Medizin I, Institut für Infektionsmedizin, Martinistrasse 52, D-20246 Hamburg, Germany.

Abstract

To evaluate the role of the polysaccharide intercellular adhesin as an energy-storage molecule, we investigated the effect of nutrient limitation on S. epidermidis biofilms. The stability of established biofilms depends on sigma(B) activity; however, the slow decay of biofilms under conditions of nutrient limitation reveal its use as an energy-storage molecule to be unlikely.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Biofilms / growth & development*
Carrier Proteins / genetics
Carrier Proteins / metabolism
Gene Expression Regulation, Bacterial*
Glucose / metabolism
Humans
Mutation
Phosphoric Monoester Hydrolases / genetics
Phosphoric Monoester Hydrolases / metabolism
Sigma Factor / metabolism*
Staphylococcus epidermidis / growth & development*

Substances

Bacterial Proteins
Carrier Proteins
RsbW protein, bacteria
SigB protein, Bacteria
Sigma Factor
Phosphoric Monoester Hydrolases
RsbU protein, Bacillus subtilis
Glucose