Genome-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga Maritima

Appl Environ Microbiol. 2005 Sep;71(9):5572-6. doi: 10.1128/AEM.71.9.5572-5576.2005.

Abstract

Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80 degrees C/0.25 h(-1), 80 degrees C/0.17 h(-1), and 85 degrees C/0.25 h(-1)) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Culture Media
  • Gene Expression Regulation, Bacterial*
  • Genome, Bacterial*
  • Hot Temperature*
  • Maltose / metabolism
  • Proteome
  • Thermotoga maritima / genetics
  • Thermotoga maritima / growth & development*
  • Thermotoga maritima / metabolism
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Culture Media
  • Proteome
  • Maltose