Apoptosis of peripheral blood lymphocytes T in nephrotic children

Ann Univ Mariae Curie Sklodowska Med. 2004;59(1):253-6.

Abstract

The purpose of the study was to assess the intensity of lymphocyte T apoptosis in nephrotic children's peripheral blood before, during and after the treatment with prednisone. The percentages of apoptotic lymphocyte T were determined by flow cytometry using annexin V. The study comprised 26 nephrotic children aged 3-14 years. The controls were 15 healthy children. In acute phase of the nephrotic syndrome before institution of the therapy the mean percentage of apoptotic lymphocytes T was 2.61%. It was significantly higher than that in controls (1.08%). During treatment with prednisone a statistically significant increase in the mean percentage of apoptotic lymphocytes T was observed. After four-week therapy with prednisone and after completion of the treatment the percentages of apoptotic lymphocytes T were 7.96% and 9.51%, respectively. They were significantly higher than those in patients before treatment and in healthy children.

MeSH terms

  • Acute Disease
  • Adolescent
  • Anti-Inflammatory Agents / therapeutic use
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Child
  • Child, Preschool
  • Female
  • Flow Cytometry
  • Humans
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / physiology
  • Lymphocyte Count
  • Male
  • Nephrotic Syndrome / drug therapy
  • Nephrotic Syndrome / immunology
  • Nephrotic Syndrome / pathology*
  • Prednisone / therapeutic use
  • Reference Values
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology*

Substances

  • Anti-Inflammatory Agents
  • Prednisone