SPINAL modulated decoupling in high field double- and triple-resonance solid-state NMR experiments on stationary samples

Neeraj Sinha; Christopher V Grant; Chin H Wu; Anna A De Angelis; Stanley C Howell; Stanley J Opella

doi:10.1016/j.jmr.2005.07.008

SPINAL modulated decoupling in high field double- and triple-resonance solid-state NMR experiments on stationary samples

J Magn Reson. 2005 Dec;177(2):197-202. doi: 10.1016/j.jmr.2005.07.008. Epub 2005 Aug 30.

Authors

Neeraj Sinha¹, Christopher V Grant, Chin H Wu, Anna A De Angelis, Stanley C Howell, Stanley J Opella

Affiliation

¹ Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, 0307 La Jolla, CA 92093-0307, USA.

PMID: 16137902
DOI: 10.1016/j.jmr.2005.07.008

Abstract

Continuous wave irradiation has limited bandwidth for heteronuclear 1H decoupling at high fields and for 13C decoupling in 1H/13C/15N triple-resonance experiments. SPINAL-16 modulation is shown to improve the efficiency of 1H and 13C heteronuclear decoupling on single crystals of peptides and on magnetically aligned samples of membrane proteins in bicelles, which is of particular importance because aqueous samples of biomolecules are lossy at high fields, which limits the strengths of the RF fields that can be applied.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacteriophages / chemistry*
Carbon Isotopes
Glycine / analogs & derivatives*
Glycine / chemistry
Hydrogen Bonding
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*
Peptides / chemistry*

Substances

Carbon Isotopes
Nitrogen Isotopes
Peptides
Glycine

Abstract

Publication types

MeSH terms

Substances

Grants and funding