Defective Fas expression exacerbates neurotoxicity in a model of Parkinson's disease

J Exp Med. 2005 Sep 5;202(5):575-81. doi: 10.1084/jem.20050163. Epub 2005 Aug 29.

Abstract

Fas (CD95), a member of the tumor necrosis factor-receptor superfamily, has been studied extensively as a death-inducing receptor in the immune system. However, Fas is also widely expressed in a number of other tissues, including in neurons. Here, we report that defects in the Fas/Fas ligand system unexpectedly render mice highly susceptible to neural degeneration in a model of Parkinson's disease. We found that Fas-deficient lymphoproliferative mice develop a dramatic phenotype resembling clinical Parkinson's disease, characterized by extensive nigrostriatal degeneration accompanied by tremor, hypokinesia, and loss of motor coordination, when treated with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at a dose that causes no neural degeneration or behavioral impairment in WT mice. Mice with generalized lymphoproliferative disease, which express a mutated Fas ligand, display an intermediate phenotype between that of lymphoproliferative and WT mice. Moreover, Fas engagement directly protects neuronal cells from MPTP/1-methyl-4-phenylpyridinium ion toxicity in vitro. Our data show that decreased Fas expression renders dopaminergic neurons highly susceptible to degeneration in response to a Parkinson-causing neurotoxin. These findings constitute the first evidence for a neuroprotective role for Fas in vivo.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aged
  • Animals
  • Brain / pathology
  • Chromatography, High Pressure Liquid
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Lymphoproliferative Disorders / metabolism*
  • MPTP Poisoning / metabolism*
  • Male
  • Mice
  • Mice, Mutant Strains
  • Middle Aged
  • Neurons / metabolism*
  • Parkinson Disease / metabolism*
  • Signal Transduction / physiology*
  • Up-Regulation*
  • fas Receptor / metabolism*

Substances

  • fas Receptor