Chromosomal number of different of somatic embryos regenerated plants were investigated in melon variety "xiaomaigua" and "Qingpilurou". Certain variations of chromosomal number were found among the regenerated plants compared with normal sample,and range of variation covered from 2n = 23-24 to 2n = 13-48 with the increase of generation,the rate from 3.3% to 30%. The results indicated that degree of variation in chromosomal number of somatic embryos regenerated melon plants increased with the time of culture, and those cultured in one to two months had the least variation. It was also found that degree of chromosomal number variations varied with melon varieties.