Abstract
In a previous work, we demonstrated that the Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. In this study, the orthologous genes from Mycobacterium smegmatis mc(2)155 were inactivated and mutants analyzed. Rv2358 protein was purified and found to bind upstream of the Rv2358 gene. Binding was inhibited by Zn(2+) ions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Gene Deletion*
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Molecular Sequence Data
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Mycobacterium smegmatis / genetics*
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Mycobacterium smegmatis / metabolism
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / metabolism
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Operon / physiology
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Promoter Regions, Genetic / physiology
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Protein Binding / genetics
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Zinc / metabolism*
Substances
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Bacterial Proteins
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Transcription Factors
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Zinc