Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells

Science. 1992 Jun 5;256(5062):1448-52. doi: 10.1126/science.1604321.

Abstract

Vertebrate T cells express either an alpha beta or gamma delta T cell receptor (TCR). The developmental relatedness of the two cell types is unresolved. alpha beta + T cells respond to specific pathogens by collaborating with immunoglobulin-producing B cells in distinct lymphoid organs such as the spleen and Peyer's patches. The precise influence of alpha beta + T cells on B cell development is poorly understood. To investigate the developmental effects of alpha beta + T cells on B cells and gamma delta + T cells, mice homozygous for a disrupted TCR alpha gene were generated. The homozygotes showed elimination of alpha beta + T cells and the loss of thymic medullae. Despite this, gamma delta + T cells developed in normal numbers, and there was an increase in splenic B cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Blastocyst
  • Blotting, Southern
  • Chimera
  • Clone Cells
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Lymphoid Tissue / growth & development
  • Lymphoid Tissue / immunology*
  • Macromolecular Substances
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Mutant Strains
  • Peyer's Patches / immunology
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell / genetics*
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • Thymus Gland / immunology

Substances

  • Macromolecular Substances
  • Receptors, Antigen, T-Cell
  • DNA