Abstract
Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.
MeSH terms
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Acute Disease
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Animals
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Bacterial Infections / complications
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Bacterial Infections / microbiology
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Cell Line
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Child
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Child, Preschool
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Ear, Middle
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Female
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Humans
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Infant
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Male
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Metapneumovirus / genetics
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Metapneumovirus / isolation & purification*
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Otitis Media / microbiology
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Otitis Media / virology*
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Paramyxoviridae Infections / complications
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Paramyxoviridae Infections / virology*
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Polymerase Chain Reaction
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Respiratory Tract Infections / complications
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Respiratory Tract Infections / virology
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Virus Diseases / complications
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Virus Diseases / virology