The interface behavior and biocatalytic activity of superoxide dismutase at carbon nanotube

Biosens Bioelectron. 2006 Jan 15;21(7):1350-4. doi: 10.1016/j.bios.2005.05.008. Epub 2005 Jun 29.

Abstract

The interface behavior and biocatalytic activity of superoxide dismutase (SOD) were studied at carbon nanotube (CNT) surface with cyclic voltammetry (CV). The results show that SOD participates in a rapid exchange with CNT and the process is a single-electron-single-proton process. The electron-transfer coefficient was calculated to be 0.52, the electron-transfer rate constant was 1.4s(-1) and the average surface coverage was measured to be 6.93 x 10(-11)+/-4.2 x 10(-12) mol cm(-2). The bioactive measurements show that SOD keeps its bioactivity at the CNT surface. SOD's remarkable ability to catalyze the dismutation of the superoxide anion (O (2)(-)) has been demonstrated.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Catalysis
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Enzyme Activation
  • Enzymes, Immobilized / analysis
  • Enzymes, Immobilized / chemistry
  • Equipment Design
  • Equipment Failure Analysis
  • Nanotubes, Carbon / chemistry*
  • Oxygen / analysis*
  • Oxygen / chemistry*
  • Superoxide Dismutase / analysis*
  • Superoxide Dismutase / chemistry*
  • Surface Properties

Substances

  • Enzymes, Immobilized
  • Nanotubes, Carbon
  • Superoxide Dismutase
  • Oxygen