Abstract
The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arginine / chemistry
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Cell Line
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Cell Membrane / metabolism*
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Cell Membrane Permeability
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Flow Cytometry
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Fluorescent Dyes / chemistry
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Humans
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Molecular Conformation
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Peptide Nucleic Acids / chemical synthesis*
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Peptide Nucleic Acids / chemistry
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Peptides / chemical synthesis*
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Peptides / chemistry
Substances
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Fluorescent Dyes
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Peptide Nucleic Acids
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Peptides
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Arginine