Abstract
The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adaptor Proteins, Signal Transducing / analysis
-
Adaptor Proteins, Signal Transducing / genetics
-
Adaptor Proteins, Signal Transducing / metabolism*
-
Animals
-
Brain / metabolism
-
Brain Chemistry
-
Cell Cycle Proteins / metabolism
-
Cell Nucleus / chemistry
-
Cytoplasm / chemistry
-
Female
-
Male
-
Mice
-
Mice, Inbred C57BL
-
Protein Interaction Mapping
-
RNA, Messenger / analysis
-
RNA, Messenger / metabolism
-
Repressor Proteins
-
Testis / chemistry
-
Testis / metabolism
-
Tissue Distribution
-
Trans-Activators
-
Transcription Factors / analysis
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Transcription, Genetic
-
Two-Hybrid System Techniques
Substances
-
Adaptor Proteins, Signal Transducing
-
Cell Cycle Proteins
-
Mta1 protein, mouse
-
RNA, Messenger
-
Repressor Proteins
-
Sh3glb1 protein, mouse
-
Spag5 protein, mouse
-
Trans-Activators
-
Transcription Factors