Elevated immunoglobulin levels in the cerebrospinal fluid from lupus-prone mice

J Neuroimmunol. 2005 Aug;165(1-2):104-13. doi: 10.1016/j.jneuroim.2005.04.022.

Abstract

The systemic autoimmune disease lupus erythematosus (SLE) is frequently accompanied by neuropsychiatric manifestations and brain lesions of unknown etiology. The MRL-lpr mice show behavioral dysfunction concurrent with progression of a lupus-like disease, thus providing a valuable model in understanding the pathogenesis of autoimmunity-induced CNS damage. Profound neurodegeneration in the limbic system of MRL-lpr mice is associated with cytotoxicity of their cerebrospinal fluid (CSF) to mature and immature neurons. We have recently shown that IgG-rich CSF fraction largely accounts for this effect. The present study examines IgG levels in serum and CSF, as well as the permeability of the blood-brain barrier in mice that differ in immune status, age, and brain morphology. In comparison to young MRL-lpr mice and age-matched congenic controls, a significant elevation of IgG and albumin levels were detected in the CSF of aged autoimmune MRL-lpr mice. Two-dimensional gel electrophoresis and MALDI-TOF MS confirmed elevation in IgG heavy and Ig light chain isoforms in the CSF. Increased permeability of the blood-brain barrier correlated with neurodegeneration (as revealed by Fluoro Jade B staining) in periventricular areas. Although the source and specificity of neuropathogenic antibodies remain to be determined, these results support the hypothesis that a breached blood-brain barrier and IgG molecules are involved in the etiology of CNS damage during SLE-like disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood-Brain Barrier / immunology
  • Cerebrospinal Fluid Proteins / cerebrospinal fluid
  • Electrophoresis, Gel, Two-Dimensional
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / cerebrospinal fluid
  • Immunoglobulin Heavy Chains / analysis
  • Immunoglobulin Light Chains / analysis
  • Immunoglobulins / biosynthesis*
  • Immunoglobulins / cerebrospinal fluid*
  • Lupus Erythematosus, Systemic / cerebrospinal fluid*
  • Lupus Erythematosus, Systemic / immunology*
  • Lupus Erythematosus, Systemic / physiopathology
  • Male
  • Mice
  • Mice, Inbred MRL lpr
  • Neurodegenerative Diseases / cerebrospinal fluid
  • Neurodegenerative Diseases / immunology
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Cerebrospinal Fluid Proteins
  • Immunoglobulin G
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulins