Longitudinal alteration of circulating dendritic cell subsets and its correlation with steroid treatment in patients with severe acute respiratory syndrome

Clin Immunol. 2005 Sep;116(3):225-35. doi: 10.1016/j.clim.2005.04.015.

Abstract

In this study, we found that 74 patients with severe acute respiratory syndrome (SARS) exhibited a rapid, dramatic decrease in numbers of circulating myeloid and plasmacytoid dendritic cells (mDCs and pDCs) during the first 2 weeks of illness (5.3- and 28.4-fold reductions for mDCs and pDCs compared with 25 healthy individuals, respectively), with slow return to normal cell numbers during convalescence (weeks 5-7 of illness on average). In addition, numbers of circulating CD4 and CD8 T cells exhibited milder reductions (2.1- and 1.8-fold at week 1) and earlier return to normal at a mean of weeks 3 and 4, respectively. A significant inverse correlation was found between numbers of DC and T-cell subsets and high-dose steroid treatment. Our novel findings thus suggest that the acute SARS-coronavirus infection probably contributes to the initial reduction of DC and T-cell subsets in blood, and that high-dose steroid administration may subsequently exacerbate and prolong low expression of the cell subsets. These findings will aid the framing of further studies of the immunopathogenesis of SARS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Hormones / therapeutic use*
  • Adult
  • Angiotensin-Converting Enzyme 2
  • Antibodies, Viral / blood
  • Base Sequence
  • Blood Cell Count
  • Carboxypeptidases / metabolism
  • Dendritic Cells / classification
  • Dendritic Cells / drug effects*
  • Dendritic Cells / enzymology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Peptidyl-Dipeptidase A
  • RNA, Viral / blood
  • RNA, Viral / genetics
  • Ribavirin / therapeutic use
  • Severe Acute Respiratory Syndrome / drug therapy*
  • Severe Acute Respiratory Syndrome / immunology*
  • Severe Acute Respiratory Syndrome / virology
  • Severe acute respiratory syndrome-related coronavirus / genetics
  • Severe acute respiratory syndrome-related coronavirus / immunology
  • T-Lymphocyte Subsets / enzymology
  • T-Lymphocyte Subsets / immunology
  • Time Factors

Substances

  • Adrenal Cortex Hormones
  • Antibodies, Viral
  • RNA, Viral
  • Ribavirin
  • Carboxypeptidases
  • Peptidyl-Dipeptidase A
  • ACE2 protein, human
  • Angiotensin-Converting Enzyme 2