Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction

Carbohydr Res. 2005 Aug 15;340(11):1914-7. doi: 10.1016/j.carres.2005.05.005.

Abstract

A new method to determine the degree of deacetylation (DD) of alpha-chitin and chitosan in the range of 17-94% DD using X-ray powder diffraction (XRD) is proposed. The results were calibrated using (1)H NMR spectroscopy for chitosan and FTIR for chitin, in comparison with the potentiometric titration method. The results showed a good linear correlation between the CrI020 from XRD and the calibrated DD value. This method is found to be simple, rapid and nondestructive to the sample.

MeSH terms

  • Acetylation
  • Animals
  • Calibration
  • Carbohydrate Conformation
  • Chitin / chemistry*
  • Chitosan / chemistry*
  • Magnetic Resonance Spectroscopy
  • Powder Diffraction
  • Spectroscopy, Fourier Transform Infrared
  • Temperature
  • X-Ray Diffraction / methods*

Substances

  • Chitin
  • Chitosan