Abstract
Peptide microcin J25 (MccJ25) inhibits bacterial RNA polymerase. We show that thermolysin-cleaved MccJ25 and MccJ25 lacking amino acids 13 to 17 also inhibit transcription. Our data and structural analysis of intact and thermolysin-digested MccJ25 suggest that distinct regions of MccJ25 are involved in transcription inhibition and cell entry.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacteriocins / chemistry*
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Bacteriocins / metabolism*
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DNA-Directed RNA Polymerases / metabolism*
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Molecular Sequence Data
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Protein Structure, Tertiary
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Structure-Activity Relationship
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Thermolysin
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Transcription, Genetic
Substances
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Bacteriocins
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Escherichia coli Proteins
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microcin
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DNA-Directed RNA Polymerases
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Thermolysin