New features in the genus Ilarvirus revealed by the nucleotide sequence of Fragaria chiloensis latent virus

Virus Res. 2005 Sep;112(1-2):32-7. doi: 10.1016/j.virusres.2005.02.010.

Abstract

Fragaria chiloensis latent virus (FClLV), a member of the genus Ilarvirus was first identified in the early 1990s. Double-stranded RNA was extracted from FClLV infected plants and cloned. The complete nucleotide sequence of the virus has been elucidated. RNA 1 encodes a protein with methyltransferase and helicase enzymatic motifs while RNA 2 encodes the viral RNA dependent RNA polymerase and an ORF, that shares no homology with other Ilarvirus genes. RNA 3 codes for movement and coat proteins and an additional ORF, making FClLV possibly the first Ilarvirus encoding a third protein in RNA 3. Phylogenetic analysis reveals that FClLV is most closely related to Prune dwarf virus, the type member of subgroup 4 of the Ilarvirus genus. FClLV is also closely related to Alfalfa mosaic virus (AlMV), a virus that shares many properties with Ilarviruses . We propose the reclassification of AlMV as a member of the Ilarvirus genus instead of being a member of a distinct genus.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence*
  • Cloning, Molecular
  • Fragaria / microbiology*
  • Ilarvirus / classification*
  • Ilarvirus / genetics
  • Ilarvirus / isolation & purification
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology*
  • Sequence Analysis, DNA