Production of active pigment epithelium-derived factor inE. coli

Biotechnol Lett. 2005 Mar;27(6):403-7. doi: 10.1007/s10529-005-1549-8.

Abstract

Human pigment epithelium-derived factor (PEDF), a neurotrophic factor, is the most potent natural inhibitor of angiogenesis. To produce the active PEDF, the gene coding for the human PEDF protein was expressed in E. coli. The rPEDF protein was expressed at 457 mg l-1 as a soluble protein. The yield of purified GST fusion protein was 14 mg l-1. Purified rPEDF inhibited tube formation in endothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Escherichia coli / genetics*
  • Eye Proteins / genetics*
  • Eye Proteins / metabolism
  • Eye Proteins / pharmacology*
  • Humans
  • Molecular Sequence Data
  • Nerve Growth Factors / genetics*
  • Nerve Growth Factors / metabolism
  • Nerve Growth Factors / pharmacology*
  • Protein Engineering / methods*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Serpins / genetics*
  • Serpins / metabolism
  • Serpins / pharmacology*

Substances

  • Eye Proteins
  • Nerve Growth Factors
  • Recombinant Proteins
  • Serpins
  • pigment epithelium-derived factor