Purification of carbon-11 PET radiotracers from unlabeled precursors by preparative HPLC and SPE

Qi-Huang Zheng; Bruce H Mock

doi:10.1002/bmc.494

Purification of carbon-11 PET radiotracers from unlabeled precursors by preparative HPLC and SPE

Biomed Chromatogr. 2005 Nov;19(9):671-6. doi: 10.1002/bmc.494.

Authors

Qi-Huang Zheng¹, Bruce H Mock

Affiliation

¹ Department of Radiology, Indiana University School of Medicine, 1345 West 16th Street, L-3 Room 202, Indianapolis, IN 46202-2111, USA. qzheng@iupui.edu

PMID: 15803445
DOI: 10.1002/bmc.494

Abstract

A general methodology for the rapid purification of carbon-11 positron emission tomography (PET) radiotracers from radiolabeling reaction mixtures has been developed. Preparative HPLC and solid-phase extraction (SPE) techniques are described which can separate some commonly used radiopharmaceuticals such as [(11)C]raclopride, [(11)C]beta-CFT and [(11)C]choline from their unlabeled precursors.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Carbon Radioisotopes / chemistry
Carbon Radioisotopes / isolation & purification*
Chemical Fractionation / methods
Choline / chemistry
Chromatography, High Pressure Liquid / methods*
Cocaine / analogs & derivatives
Cocaine / chemistry
Positron-Emission Tomography
Raclopride / chemistry
Radiopharmaceuticals / chemical synthesis
Radiopharmaceuticals / chemistry
Radiopharmaceuticals / isolation & purification*

Substances

Carbon Radioisotopes
Radiopharmaceuticals
Raclopride
(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
Cocaine
Choline