Abstract
The occurrence of 7 beta,8 alpha-dihydroxy-(9 alpha,10 alpha)-epoxy-7,8,9,10- tetrahydrobenzo(a)pyrene (BPDE-)-DNA adducts was investigated by synchronous fluorescence spectrophotometry in human pulmonary alveolar macrophages (PAM) collected by bronchoalveolar lavage. In preliminary assays BPDE-DNA adducts were consistently detected in PAM of smokers, whereas samples from nonsmokers did not exhibit specific peaks.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / analysis*
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Adult
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Aged
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Bronchoalveolar Lavage Fluid / metabolism
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DNA / analysis*
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DNA Adducts*
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Female
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Humans
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Macrophages, Alveolar / metabolism*
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Male
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Middle Aged
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Respiratory Tract Diseases / metabolism
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Spectrometry, Fluorescence
Substances
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DNA Adducts
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benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA
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7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
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DNA