Molecular mechanisms by which rRNA mutations confer resistance to clindamycin

Jacob Poehlsgaard; Peter Pfister; Erik C Böttger; Stephen Douthwaite

doi:10.1128/AAC.49.4.1553-1555.2005

Molecular mechanisms by which rRNA mutations confer resistance to clindamycin

Antimicrob Agents Chemother. 2005 Apr;49(4):1553-5. doi: 10.1128/AAC.49.4.1553-1555.2005.

Authors

Jacob Poehlsgaard¹, Peter Pfister, Erik C Böttger, Stephen Douthwaite

Affiliation

¹ Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. jacobp@bmb.sdu.dk

Abstract

The mechanisms by which rRNA mutations confer clindamycin resistance were examined in Mycobacterium smegmatis strains containing homogeneous populations of ribosomes with base substitutions at nucleotides A2058 and A2059. Computer graphic predictions based on structural studies correlate with the resistance phenotypes for six of seven strains with unique rRNA mutations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Clindamycin / pharmacology*
Drug Resistance, Bacterial / genetics*
Microbial Sensitivity Tests
Models, Molecular
Mutation*
Mycobacterium smegmatis / drug effects*
Mycobacterium smegmatis / genetics
RNA, Ribosomal / genetics*
RNA, Ribosomal, 23S / chemistry
RNA, Ribosomal, 23S / genetics

Substances

Anti-Bacterial Agents
RNA, Ribosomal
RNA, Ribosomal, 23S
Clindamycin