Loss of heterozygosity at chromosomes 3p and 17p in primary non-small cell lung cancer

Magdalena Chmara; Agnieszka Wozniak; Karolina Ochman; Grazyna Kobierska; Rafal Dziadziuszko; Katarzyna Sosinska-Mielcarek; Ewa Jassem; Jan Skokowski; Jacek Jassem; Janusz Limon

Loss of heterozygosity at chromosomes 3p and 17p in primary non-small cell lung cancer

Anticancer Res. 2004 Nov-Dec;24(6):4259-63.

Authors

Magdalena Chmara¹, Agnieszka Wozniak, Karolina Ochman, Grazyna Kobierska, Rafal Dziadziuszko, Katarzyna Sosinska-Mielcarek, Ewa Jassem, Jan Skokowski, Jacek Jassem, Janusz Limon

Affiliation

¹ Department of Biology and Genetics, Medical University of Gdansk, Gdansk, Poland.

PMID: 15736482

Abstract

Background: Loss of heterozygosity (LOH) of selected regions at chromosomes 3p and 17p in non-small cell lung cancer (NSCLC) and the association of these abnormalities with major clinical parameters and prognosis were studied.

Materials and methods: The study group included 92 consecutive primary NSCLC tumours and four microsatellite markers from chromosome 3p and three markers from 17p were analyzed.

Results: LOH of at least one locus was found in 83% of all analyzed tumours. Most frequently deletion (58%) was found at locus D3S1481 (3p14.2). Sequence deletions of D17S520 (17p12) and TP53 (17p13.1) occurred in 52% of tumours. LOH occurrence at 3p and 17p was more frequent in squamous cell carcinomas compared to adenocarcinomas (89% vs. 75%), but this difference was not significant.

Conclusion: No significant association was found between LOH on any analyzed loci and tumour stage (TNM) and grade (G). There was no correlation between LOH and survival.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Non-Small-Cell Lung / genetics*
Chromosomes, Human, Pair 17 / genetics*
Chromosomes, Human, Pair 3 / genetics*
Female
Humans
Loss of Heterozygosity*
Lung Neoplasms / genetics*
Male