Objective: To explore the molecular mechanism of cell response induced by hydroquinone, we investigated the protein profile after treatment with hydroquinone in human embryonic lung fibroblasts.
Methods: After HQ treatment, cells were lysed in lysis solution, then the supernatant were collected and precipitated with acetone prior to protein concentration determination. The total cellular proteins were separated using two-dimensional gel electrophoresis and visualized by colloidal coomassie blue staining. Digital images were analyzed using Imagemaster 2.0 software. The differentially expressed protein spots were picked and digestion in gel then identified by peptide mass fingerprinting using MALDI-TOF.
Results: There were 15 protein spots changed after HQ stimulation. Among them 8 protein spots were identified by PMF including some oxidative stress and cytoskeleton related proteins.
Conclusion: Protein profile was altered after HQ stimulation.