Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

J Gen Virol. 2005 Mar;86(Pt 3):719-725. doi: 10.1099/vir.0.80546-0.

Abstract

Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl and a teal during virological surveillance in Guangdong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order that is typical of avian coronaviruses. The spike, membrane and nucleocapsid protein genes of pf/CH/LKQ3/03 had >99 % identity to those of the avian infectious bronchitis coronavirus H120 vaccine strain (Massachusetts serotype) and other Massachusetts serotype isolates. Furthermore, when pf/CH/LKQ3/03 was inoculated experimentally into chickens (specific-pathogen-free), no disease signs were apparent. tl/CH/LDT3/03 had a spike protein gene with 95 % identity to that of a Chinese infectious bronchitis virus (IBV) isolate, although more extensive sequencing revealed the possibility that this strain may have undergone recombination. When inoculated into chickens, tl/CH/LDT3/03 resulted in the death of birds from nephritis. Taken together, this information suggests that pf/CH/LKQ3/03 might be a revertant, attenuated vaccine IBV strain, whereas tl/CH/LDT3/03 is a nephropathogenic field IBV strain, generated through recombination. The replication and non-pathogenic nature of IBV in domestic peafowl and teal under field conditions raises questions as to the role of these hosts as carriers of IBV and the potential that they may have to transmit virus to susceptible chicken populations.

MeSH terms

  • Animals
  • Birds / virology*
  • Chickens
  • Coronavirus Infections / prevention & control
  • Coronavirus Infections / veterinary*
  • Coronavirus Infections / virology
  • Genome, Viral
  • Infectious bronchitis virus / immunology
  • Infectious bronchitis virus / isolation & purification*
  • Molecular Sequence Data
  • Phylogeny
  • Poultry Diseases / virology*
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Viral

Associated data

  • GENBANK/AY702085
  • GENBANK/AY702975