Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein

Qiang Yun; Ting Chen; Guifeng Zhang; Jingxiu Bi; Guanghui Ma; Zhiguo Su

doi:10.1007/s10529-004-8354-7

Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein

Biotechnol Lett. 2005 Feb;27(3):213-7. doi: 10.1007/s10529-004-8354-7.

Authors

Qiang Yun¹, Ting Chen, Guifeng Zhang, Jingxiu Bi, Guanghui Ma, Zhiguo Su

Affiliation

¹ National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.

PMID: 15717132
DOI: 10.1007/s10529-004-8354-7

Abstract

A novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Gel
Granulocyte Colony-Stimulating Factor / chemistry
Granulocyte Colony-Stimulating Factor / isolation & purification
Humans
Interferon Type I / analysis
Interferon Type I / chemistry
Molecular Structure
Molecular Weight
Polyethylene Glycols / chemistry*
Recombinant Proteins / chemical synthesis
Recombinant Proteins / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Interferon Type I
Recombinant Proteins
Granulocyte Colony-Stimulating Factor
Polyethylene Glycols
monomethoxypolyethylene glycol