[Purification and relative molecular mass determination of tea glycoprotein by high performance liquid chromatography-electrospray ionisation mass spectrometry]

Peng Zhou; Mingyong Xie; Yuanxing Wang

[Purification and relative molecular mass determination of tea glycoprotein by high performance liquid chromatography-electrospray ionisation mass spectrometry]

Se Pu. 2004 Jan;22(1):27-9.

[Article in Chinese]

Authors

Peng Zhou¹, Mingyong Xie, Yuanxing Wang

Affiliation

¹ Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China.

PMID: 15712939

Abstract

Tea glycoprotein (TGC) was extracted from coarse tea leaves produced in Jiangxi Province. The separation and purification were carried out by using gel chromatography and high performance liquid chromatography. Furthermore, relative molecular mass (M(r)) of TGC was determined by gel chromatography and high performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS), respectively. It is 51 500.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Electrophoresis, Capillary / methods
Glycoproteins / chemistry*
Glycoproteins / isolation & purification
Molecular Weight
Spectrometry, Mass, Electrospray Ionization / methods
Tea / chemistry*

Substances

Glycoproteins
Tea