High level expression of recombinant chicken interferon-alpha using baculovirus

J Vet Med Sci. 2005 Jan;67(1):25-8. doi: 10.1292/jvms.67.25.

Abstract

Bioactive recombinant chicken interferon-alpha (ChIFN-alpha) was expressed in a baculovirus system. For easy purification, it was expressed as ChIFN-alpha bearing histidine hexamer (His-tag) at C-terminal, designated ChIFN-alphaHis. The expressed proteins were detected by SDS-PAGE analysis with Coomassie brilliant blue staining as around 23 and 19 kDa bands thought to be immature and matured ChIFN-alphaHis respectively. The purified ChIFN-alphaHis with a nickel chelated column showed anti-viral activity in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology
  • Baculoviridae / metabolism*
  • Cell Line
  • Chick Embryo
  • Chickens
  • Cloning, Molecular / methods
  • Gene Expression
  • Genetic Vectors
  • Interferon Type I / biosynthesis*
  • Interferon Type I / pharmacology
  • Recombinant Proteins
  • Spodoptera

Substances

  • Antiviral Agents
  • Interferon Type I
  • Recombinant Proteins