Objective: To investigate whether polymorphism in gene for surfactant protein A(SPA) has any bearing on individual susceptibility to the development of chronic obstructive pulmonary disease(COPD).
Methods: The genotypes of 88 patients with COPD and 87 healthy smoking subjects as controls were tested with polymerase chain reaction followed by restriction fragment length polymorphism analysis for SPA gene.
Results: In COPD group, the frequencies of +186 locus genotypes AA, AG and GG were 86.4%, 12.5% and 1.1%i respectively and in the control group, these were 66.7%, 27.6% and 5.7%; the frequencies of polymorphic genotypes or alleles showed statistically significant difference between the COPD group and the control group(P<0.05). The frequencies of polymorphic genotypes at +655 locus and +667 loci showed no significant difference between the COPD group and the control group in(P>0.05).
Conclusion: Genetic polymorphism in SPA is associated with the development of COPD in the Chinese Hans.