Identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR

Manfred Weidmann; P Schmidt; M Vackova; K Krivanec; P Munclinger; F T Hufert

doi:10.1128/JCM.43.2.808-812.2005

Identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR

J Clin Microbiol. 2005 Feb;43(2):808-12. doi: 10.1128/JCM.43.2.808-812.2005.

Authors

Manfred Weidmann¹, P Schmidt, M Vackova, K Krivanec, P Munclinger, F T Hufert

Affiliation

¹ Unit for Microbiological Preparedness and Emerging Infectious Diseases, University Hospital, Stefan-Meier-Str. 19, 79014 Freiburg, Germany. manfred.weidmann@uniklinik-freiburg.de

Abstract

A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arvicolinae
Hantavirus Infections / veterinary*
Hantavirus Infections / virology
Mice
Molecular Sequence Data
Muridae / virology*
Orthohantavirus / classification*
Orthohantavirus / genetics*
Orthohantavirus / isolation & purification
Random Amplified Polymorphic DNA Technique
Reverse Transcriptase Polymerase Chain Reaction / methods*
Rodent Diseases / transmission*
Rodent Diseases / virology
Sequence Analysis, DNA
Taq Polymerase / metabolism

Substances

Taq Polymerase

Associated data

GENBANK/AJ578081
GENBANK/AJ578082