In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury

Mem Inst Oswaldo Cruz. 2004 Nov;99(7):697-701. doi: 10.1590/s0074-02762004000700006. Epub 2005 Jan 12.

Abstract

Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Cardiovascular Diseases / diagnosis*
  • Cardiovascular Diseases / enzymology
  • Cardiovascular Diseases / pathology
  • Cell Culture Techniques
  • Clinical Enzyme Tests*
  • Creatine Kinase / analysis*
  • Creatine Kinase, MB Form
  • Creatine Kinase, MM Form
  • Isoenzymes / analysis
  • L-Lactate Dehydrogenase / analysis*
  • Mice
  • Microscopy, Electron, Transmission
  • Myocytes, Cardiac / enzymology*
  • Myocytes, Cardiac / ultrastructure
  • Sensitivity and Specificity

Substances

  • Biomarkers
  • Isoenzymes
  • L-Lactate Dehydrogenase
  • Creatine Kinase
  • Creatine Kinase, MB Form
  • Creatine Kinase, MM Form