Insight into pollutant bioavailability and toxicity using Raman confocal microscopy

J Microbiol Methods. 2005 Mar;60(3):417-22. doi: 10.1016/j.mimet.2004.10.016.

Abstract

Raman confocal microscopy was used to discriminate between cultures of Burkholderia xenovorans LB400 exposed to four different common environmental pollutants: phenanthrene, dodecane, 3-chlorobiphenyl and pentachlorophenol. Evidence is presented for the application of Raman spectroscopy as a bioassay for pollutant bioavailability and toxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkanes / pharmacokinetics
  • Alkanes / toxicity
  • Biological Availability
  • Biphenyl Compounds / pharmacokinetics
  • Biphenyl Compounds / toxicity
  • Burkholderia / drug effects*
  • Burkholderia / metabolism*
  • Environmental Pollutants / pharmacokinetics*
  • Environmental Pollutants / toxicity*
  • Microscopy, Confocal
  • Organic Chemicals / pharmacokinetics*
  • Organic Chemicals / toxicity*
  • Pentachlorophenol / pharmacokinetics
  • Pentachlorophenol / toxicity
  • Phenanthrenes / pharmacokinetics
  • Phenanthrenes / toxicity
  • Principal Component Analysis
  • Spectrum Analysis, Raman

Substances

  • Alkanes
  • Biphenyl Compounds
  • Environmental Pollutants
  • Organic Chemicals
  • Phenanthrenes
  • n-dodecane
  • phenanthrene
  • Pentachlorophenol
  • 3-chlorobiphenyl