Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, 2-ethyl-5-methyl-3,3-diphenylpyraline and methadol in meconium by liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 25;814(2):369-73. doi: 10.1016/j.jchromb.2004.10.068.

Abstract

This paper details a validated liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS) method for the quantification of methadone, and its metabolites 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), 2-ethyl-5-methyl-3,3-diphenylpyraline (EMDP) and methadol in human meconium. Limits of detection (LOD) were determined to be 1.0 ng/g for methadone, EDDP and EMDP and 2.5 ng/g for methadol. The limits of quantitation (LOQ) for methadone, EDDP, EMDP were 5 and 25 ng/g for methadol. Linearity ranged from 5.0 to 500 ng/g. Following solid-phase extraction, no matrix effect was observed. This method proved to be suitable for the quantification of methadone, EDDP and EMDP and the semi-quantitation of methadol in meconium. Literature review revealed no other published LC-APCI-MS/MS method for the detection of methadone and its three main metabolites in meconium specimens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • Atmospheric Pressure
  • Humans
  • Mass Spectrometry / methods*
  • Meconium / chemistry*
  • Methadone / analysis*
  • Pyrrolidines / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • 2-ethyl-5-methyl-3,3-diphenylpyrroline
  • Pyrrolidines
  • Methadone
  • 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine